anticancer effect of bovine lactoferrin on human esophagus cancer cell line
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abstract
background: lactoferrin (lf) is a glycoprotein, a member of the transferrin family.from ten known mechanisms of anti-cancer chemoperotecive compounds, lf alone, has six of these functions and inhibits cancer. in this study, the effect of lactoferrin purified from bovine colostrum was studied as an anti-cancer agent on esophageal cancer cell line. materials and methods: bovine colostrum were collected immediately after giving birth. at first, the fat, casein, and some of the milk proteins were removed. then, lactoferrin was purified using cm-sephadex-c50 cation exchange chromatography by fplc system. purified lactoferrin with 80 kda molecular weight and 2mg/ml concentration was obtained. esophageal cancer cell line kyse-30 and normal cell line hek were cultured. after appropriate confluency, different concentrations of lf were added to kyse-30 and hek for 20 h and its anti-cancer effect was evaluated by mtt and flow cytometric methods. the maximum concentration inhibitory effect was studied at different times using mtt method. results: mtt test determined that 500 µg/ml of lactoferrin reduced cell viability in esophageal cancer cell lines kyse by 53% and 80% after 20 and 62 hours, respectively, but had no effect on normal cells. also, flow cytometric analysis determined that lactoferrin was able to induce apoptosis in kyse-30 cell line. conclusion: the isolated lactoferrin from bovine milk showed inhibitory effect on esophageal cancer cell line whereas; it did not have any significant effect on normal cells.
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Corresponding Author: Fatemeh Moradian Assistant professor of Biochemistry, Basic Sciences Group, Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran, Iran, P.O.B. 578. Telefax:+98-11-33687655 E-mail: [email protected] & [email protected] Abstract Background: Lactoferrin is a glycoprotein with a molecular weight of 80 kDa, as a member of the transferring family. In...
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research in molecular medicineجلد ۴، شماره ۱، صفحات ۱۸-۲۳
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